This paper demonstrates the utility of a universal calibration approach for liquid permeation testing using flame ionization detectors. ASTM F 739 is a well-known method for determining the resistance of gloves and other protective clothing materials to permeation by liquids under conditions of continuous contact. Due to the wide array of chemicals that workers can be exposed to, there is great interest in permeation data for hundreds of different chemicals. The flame ionization detector (FID), considered to be one of the most universal detectors, is a logical choice for analyzing various types of organic compounds. The universal calibration method proposed in this paper relies on the principal that the number of ionic intermediates produced as a result of an organic sample being pyrolyzed is nearly proportional to the number of carbon atoms in the sample. To verify this calibration method for a wide range of conditions, systematic experiments have been carried out to study the effect of flow rate of the calibration gas, the independence of functional groups such as alcohols, amines, and halogens, and the effect of detector optimization on linearity.